Belinal® has a Unique Lignan Standard
Belinal® extract is produced using a proprietary extraction process that does not involve alcohol or other organic solvents. We believe that pure natural ingredients are essential for the therapeutic potential of polyphenols, and our research confirms that the effects of Belinal® are truly remarkable.
Belinal® is characterized by a distinctive lignan fingerprint.
Figure 1. Typical Chromatogram of Belinal® with Identified 6 Lignans
Quantification of Lignans in Belinal® – Silver Fir Branch Extract
The compounds present in the Silver Fir branch extract Belinal® were identified and quantified based on their MS fragmentation patterns, high resolution mass measurements, ultraviolet (UV) spectra, retention times, and comparison with reference standard compounds (Table 1; Figure 1). Six lignans were identified, representing the six highest peaks on the HPLC chromatogram, and together they define the standard of the Silver Fir branch extract Belinal®.
Table 2. Quantification of Standard lignans in Belinal®
| Compound | Elemental
Composition |
m/z (ESI(-)) | % (g compound/100 g Belinal®) |
| 1 Isolariciresinol | C20H24O6 | 359 | 1.78 |
| 2 Hydroxymatairesinol | C20H22O7 | 373, 355, 327 | 0.89 |
| 3 Secoisolariciresinol | C20H26O6 | 361, 346, 315 | 5.51 |
| 4 Lariciresinol | C20H24O6 | 359 | 0.79 |
| 5 Pinoresinol | C20H22O6 | 357 | 0.24 |
| 6 Matairesinol | C20H22O6 | 357 | 0.50 |
For the identification of characteristic standard compounds in Belinal®, the HPLC method is used. Guidelines for compound identification are available upon request.
References:
Tavčar Benković E, Žigon D, Mihailović V, Petelinc T, Jamnik P, Kreft S. Identification, in vitro and in vivo Antioxidant Activity, and Gastrointestinal Stability of Lignans from Silver Fir (Abies alba) Wood Extract Belinal. J Wood Chem Technol [Internet]. 2017 Nov 2;37(6):467–77.